This research investigates the enhancement of Artificial Immune Systems (AIS) for solving the Traveling Salesman Problem (TSP) through hybridization with Neighborhood Improvement (NI) and parameter fine-tuning. Two main experiments were conducted: Experiment A identified the optimal integration points for NI within AIS, revealing that position 2 (AIS+NIpos2) improved solution quality by an average of 27.78% compared to other positions. Experiment B benchmarked AIS performance with various enhancement techniques. Using symmetric and asymmetric TSP datasets, the results showed that integrating NI at strategic points and fine-tuning parameters boosted AIS performance by up to 46.27% in some cases. The hybrid and fine-tuned version of AIS (AIS-th) consistently provided the best solution quality, with up to a 50.36% improvement, though it required more computational time. These findings emphasize the importance of strategic combinations and fine-tuning for creating effective optimization algorithms.

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